The Bhattacharyya Lab employs an integrative approach to understand the molecular mechanism for the regulation and activation of kinase signalling pathways in the context of learning and memory, and cognitive impairments in Down Syndrome.
We use single-molecule total internal reflection fluorescence (TIRF) microscopy to understand biochemical processes at a single-particle resolution. Reconstituting protein/s on functionalized glass or lipid bilayers, we aim to monitor and manipulate them one molecule at a time.
Native mass spectrometry and lipidomics
We use native mass spectrometry to investigate the macromolecular assembly and composition of proteins and their lipid milieu. This will enable us to connect protein assemblies and protein-membrane crosstalk with their functions.
We use X-ray crystallography to obtain the 3D-structure of proteins. Combining this with other biophysical and biochemical tools, we connect the structure of the protein with its conformational dynamics, and eventually function.
We use molecular dynamics (MD) simulations and network-based analysis approaches to study the conformational changes and dynamics of macromolecular assembles, with a focus on understanding allosteric communication in proteins complexes. We use these computational methods as a hypothesis generating vehicle to guide our experiments.
New tools and techniques
We are interested in developing new tools and techniques to study (1) the molecular mechanisms of signal transduction at a single-cell resolution, and (2) the effect of lipid-microenvironment composition in shaping the assembly and function of proteins.